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KMID : 0364820090450040339
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Korean Journal of Microbiology
2009 Volume.45 No. 4 p.339 ~ p.345
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Isolation, Identification, and Characterization of Weissella Strains with High Ornithine Producing Capacity from Kimchi
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Yu Jin-Ju
Park Hyoung-Ju
Kim Su-Gon
Oh Suk-Heung
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Abstract
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Two lactic acid bacteria (LAB) with high ornithine-producing capacity were isolated from kimchi. Examination of the biochemical features using an API kit indicated that the strains belonged to the members of Weissella genus. They were gram positive, short rod-type bacteria, and able to grow anaerobically with CO2 production. The isolates grew well on MRS broth at 25~37oC and pH of 6.0~7.0. The optimum temperature and pH for growth are 30oC and pH 6.5. The isolates fermented arabinose, ribose, xylose, glucose but not cellobiose, galactose, raffinose, or trehalsoe. The 16S rDNA sequences of isolates showed 99.6% and 99.7% homology with the Weissella koreensis S5623 16S rDNA (access no. AY035891). They were accordingly identified and named as Weissella koreensis OK1-4 and Weissella koreensis OK1-6, and could produce ornithine from MRS broth supplemented with 1% of arginine at a productivity of 27.01 and 31.41 mg/L/h, respectively. This is the first report on the production of ornithine by the genus Weissella isolated from kimchi.
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KEYWORD
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kimchi, ornithine, producing, Weissella koreensis
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